Information-enriched characterization of mobile populations. Morphological details from illustrations or photos can include for the richness of flow cytometry information. As an example, the figure displays an otherwise conventional apoptosis assay using Annexin V and PI, incorporating mobile imaging to characterize cells in each populace to expose morphologically unique attributes. These insights could not have already been received from multiplex staining by itself.

As samples are acquired around the Attune CytPix Flow Cytometer, the substantial-velocity brightfield camera captures and stores pictures of detected situations at a price around six,000 photos per next, depending upon the flow charge and graphic dimensions. For bigger adaptability, Attune Cytometric Computer software permits you to modify the impression capture frequency as needed and select a certain gate for graphic capture, if needed.

(B) Uncooked 264.7 macrophages tend to be annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The amount of singlets accessible for analysis raises once the consumer trains and employs a far more exact product.

Uncover new insights into your experimental info by visualizing populations working with put together fluorescence and morphology data. Our computer software interface wizards empower downsampling, concatenation (merged information) and dimensionality applications which might be built in the software program for an intuitive, streamlined details Assessment practical experience.

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Below, a seasoned person has gated singlets confidently. Immediately after evaluating the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate contains a lot more than four% aggregates.

Optimizing gating system. Even strong guide singlet gating is mistake-inclined and stays a subjective final decision place in Virtually all flow cytometry assays. Imaging may be used to substantiate and adjust gates to incorporate only solitary cells of fascination.

Our experts will structure panels to suit your needs as again and again you may need—free of charge, no product obtain necessary.

Early signs of this contamination experienced initially been dismissed as debris. Backgating to find out new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also helps you to use morphological characteristics to find out fascinating subpopulations that could not be clear from flow cytometry get more info information alone.

Probably most of all, these activities contain cells of Plainly unique phenotypes which could lead on to incorrect conclusions regarding double constructive functions (particularly in exceptional populations).

Audio waves permit exact cell alignment, leading to Extraordinary info high quality and ~10X faster sample flow prices.

We Formerly shown the power of imaging Automobile-T/Ramos cell interactions. Permit’s check out just the populace of greatest fascination, the double good gatherings, to learn more. We are able to now use prolonged morphological parameters (in this article, circularity and skewness of depth) to further analyze the features of those populations and refine gating on these activities, escalating facts robustness.

Leverage the power of Sophisticated technological know-how and innovation to speed up your discoveries. Trust from the Attune Flow Cytometer household to provide the trustworthiness, pace, and precision that set your function aside.

Versatile payment system from Agilent Money Methods permit you to obtain the vital instrumentation you would like for immediate project, or to expand your lab.

At the center of each and every Attune Flow Cytometer lies our innovative acoustic focusing technological know-how, which makes use of sound waves to meticulously align cells inside the flow cell. This modern strategy surpasses standard hydrodynamic techniques, leading to a lot quicker processing speeds, Fantastic info good quality, and increased sample throughput.

As the cell line had Earlier been analyzed to the Attune CytPix Flow Cytometer, the researchers went again to the pictures and were able to document the microbial an infection not less than 5 times before.

The Attune CytPix Flow Cytometer allows users to visualize samples in the course of acquisition, confirm the accuracy of gating strategies, and help outcomes with trusted documentation for publication purposes.

What gave the impression to be a homogeneous populace is actually a lot more diverse—an insight that should be thought of when interpreting final results.